# BacterialTyper: a bioinformatics pipeline for the integrative analysis of bacterial WGS
# Table of Contents
- [Introduction](#introduction)
- [Installation](#installation)
- [Documentation](#documentation)
- [License](#license)
- [Citation](#citation)
# Introduction
We present BacterialTyper, a pipeline for the analysis of bacterial WGS data that integrates and facilitates the interpretation of results generated from multiple software analysis. It is capable of processing and identifying bacterial strains, identifying resistance and virulence genes, and generating data for outbreak analysis using WGS data from isolated microbial cultured colonies. The design of this bioinformatic tool allows comparing samples with an internal database (previously identified samples) and external databases.
The pipeline is written in Python with a modular architecture and based on open-source software and databases engines. Multiple tasks are performed by each of several modules including: preparation of raw data; assembly and annotation; bacterial strain identification; mobile genetic elements identification (plasmids, putative pathogenicity islands or phage insertions regions); generation of a virulence and resistance profile; clustering based on sequence similarity; phylogenetic analysis; integration of metadata, etc. The tool allows to compare samples with previously identified samples (collected and internal database) but it also uses, and updates periodically, external databases from different sources.
# Installation
To install _BacterialTyper_ you need to create a _conda_ environment with all dependendies requiere. In following weeks/months a stable _conda_ recipe will be available.
We recommend using _mamba_ to solve all dependencies and conflicts. To install _mamba_ simply type in the base environemt:
```
## create environment and activate it
conda create -y -n BacterialTyper
conda activate BacterialTyper
## install mamba
conda install -c conda-forge mamba
```
Then, use mamba instead of conda to install further packages.
So far, you can either install BacterialTyper using:
- a configuration file within this git repository (**BacterialTyper/devel/conda/bacterialtyper-list.txt**). See file [here](https://github.com/HCGB-IGTP/BacterialTyper/blob/master/devel/conda/bacterialtyper-list.txt)
- or typing the following _conda_/_mamba_ command:
```
mamba install -c r -c bioconda -c conda-forge \
perl-bio-searchio-hmmer perl-bioperl \
trimmomatic fastqc spades snippy prokka kma \
agrvate staphopia-sccmec kraken2 bowtie2 \
ncbi-amrfinderplus bracken krakentools mlst
```
Finally, you will need to install _BacterialTyper_ which is host in pypi [here](https://pypi.org/project/BacterialTyper/). Use _pip_ and type:
```
pip install BacterialTyper
```
This will create a working installation of _BacterialTyper_ and all dependencies.
Verify the configuration using
```
BacterialTyper config check
```
# Documentation
For a full documentation and details visit Read the Docs site [here](https://bacterialtyper.readthedocs.io/en/latest/index.html).
# License
MIT License
Copyright (c) 2020-2022 HCGB-IGTP
See additional details [here](./LICENSE)
# Citation
BacterialTyper was initially described in the ECCMID 2020 Poster [1]. See and example of usage in the characterization of some clinical samples [2].
[1] Sanchez-Herrero, J.; Lacoma, A.; Molina-Moya, B.; Giménez, M.; Molinos, S.; Prat, C.; Sumoy, L. BacterialTyper: A Bioinformatics Pipeline for the Integrative Analysis of Bacterial Whole Genome Sequencing. Abstract 1981. In Proceedings of the 30th European Congress of Clinical Microbiology and Infectious Diseases, Paris, France, 18–21 April 2020
[2] A. Lacoma et al., “Genotypic and Phenotypic Characterization of Staphylococcus aureus Isolates from the Respiratory Tract in Mechanically-Ventilated Patients,” Toxins (Basel)., vol. 13, no. 2, p. 122, Feb. 2021.
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"description": "# BacterialTyper: a bioinformatics pipeline for the integrative analysis of bacterial WGS \n\n# Table of Contents\n\n- [Introduction](#introduction)\n- [Installation](#installation)\n- [Documentation](#documentation)\n- [License](#license)\n- [Citation](#citation)\n\n# Introduction\n\nWe present BacterialTyper, a pipeline for the analysis of bacterial WGS data that integrates and facilitates the interpretation of results generated from multiple software analysis. It is capable of processing and identifying bacterial strains, identifying resistance and virulence genes, and generating data for outbreak analysis using WGS data from isolated microbial cultured colonies. The design of this bioinformatic tool allows comparing samples with an internal database (previously identified samples) and external databases. \n\nThe pipeline is written in Python with a modular architecture and based on open-source software and databases engines. Multiple tasks are performed by each of several modules including: preparation of raw data; assembly and annotation; bacterial strain identification; mobile genetic elements identification (plasmids, putative pathogenicity islands or phage insertions regions); generation of a virulence and resistance profile; clustering based on sequence similarity; phylogenetic analysis; integration of metadata, etc. The tool allows to compare samples with previously identified samples (collected and internal database) but it also uses, and updates periodically, external databases from different sources. \n\n![Workflow](docs/source/images/workflow/all.jpg \"BacterialTyper pipeline\")\n\n# Installation\n\nTo install _BacterialTyper_ you need to create a _conda_ environment with all dependendies requiere. In following weeks/months a stable _conda_ recipe will be available. \n\nWe recommend using _mamba_ to solve all dependencies and conflicts. To install _mamba_ simply type in the base environemt:\n\n```\n## create environment and activate it\nconda create -y -n BacterialTyper\nconda activate BacterialTyper\n\n## install mamba\nconda install -c conda-forge mamba\n```\n\nThen, use mamba instead of conda to install further packages.\n\nSo far, you can either install BacterialTyper using:\n- a configuration file within this git repository (**BacterialTyper/devel/conda/bacterialtyper-list.txt**). See file [here](https://github.com/HCGB-IGTP/BacterialTyper/blob/master/devel/conda/bacterialtyper-list.txt)\n- or typing the following _conda_/_mamba_ command:\n\n```\nmamba install -c r -c bioconda -c conda-forge \\\n perl-bio-searchio-hmmer perl-bioperl \\\n trimmomatic fastqc spades snippy prokka kma \\\n agrvate staphopia-sccmec kraken2 bowtie2 \\\n ncbi-amrfinderplus bracken krakentools mlst\n```\n\nFinally, you will need to install _BacterialTyper_ which is host in pypi [here](https://pypi.org/project/BacterialTyper/). Use _pip_ and type:\n\n```\npip install BacterialTyper\n```\n\nThis will create a working installation of _BacterialTyper_ and all dependencies.\n\nVerify the configuration using \n\n```\nBacterialTyper config check\n```\n\n# Documentation\n\nFor a full documentation and details visit Read the Docs site [here](https://bacterialtyper.readthedocs.io/en/latest/index.html).\n\n# License\n\nMIT License\n\nCopyright (c) 2020-2022 HCGB-IGTP\n\nSee additional details [here](./LICENSE)\n\n# Citation\n\nBacterialTyper was initially described in the ECCMID 2020 Poster [1]. See and example of usage in the characterization of some clinical samples [2].\n\n[1] Sanchez-Herrero, J.; Lacoma, A.; Molina-Moya, B.; Gim\u00e9nez, M.; Molinos, S.; Prat, C.; Sumoy, L. BacterialTyper: A Bioinformatics Pipeline for the Integrative Analysis of Bacterial Whole Genome Sequencing. Abstract 1981. In Proceedings of the 30th European Congress of Clinical Microbiology and Infectious Diseases, Paris, France, 18\u201321 April 2020\n\n[2] A. Lacoma et al., \u201cGenotypic and Phenotypic Characterization of Staphylococcus aureus Isolates from the Respiratory Tract in Mechanically-Ventilated Patients,\u201d Toxins (Basel)., vol. 13, no. 2, p. 122, Feb. 2021.\n\n\n",
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