| Name | FlowCyPy JSON |
| Version |
0.27.9
JSON |
| download |
| home_page | None |
| Summary | A package for light scattering computation. |
| upload_time | 2025-07-09 01:55:13 |
| maintainer | None |
| docs_url | None |
| author | None |
| requires_python | >=3.10 |
| license | MIT License
Copyright (c) 2020 Martin de Sivry
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
|
| keywords |
flow cytometry
light scattering
mie theory
backscatter
rayleigh scattering
biophotonics
nanoparticle
microfluidics
fluorescence
biomedical imaging
spectroscopy
optical simulation
machine learning
signal processing
photonics
cytometry
optical sensors
laser scattering
particle detection
flow analysis
|
| VCS |
|
| bugtrack_url |
|
| requirements |
No requirements were recorded.
|
| Travis-CI |
No Travis.
|
| coveralls test coverage |
No coveralls.
|
|logo|
FlowCyPy: Flow Cytometer Simulation Tool
========================================
.. list-table::
:widths: 10 25 25 25
:header-rows: 0
* - Meta
- |python|
- |docs|
-
* - Testing
- |ci/cd|
- |coverage|
- |colab|
* - PyPi
- |PyPi|
- |PyPi_download|
-
* - Anaconda
- |anaconda|
- |anaconda_download|
- |anaconda_date|
Overview
--------
**FlowCyPy** is a cutting-edge Python library designed to simulate flow cytometer experiments. By generating realistic Forward Scatter (FSC) and Side Scatter (SSC) signals, FlowCyPy enables detailed modeling of flow cytometry setups, making it ideal for researchers and engineers working with extracellular vesicles (EVs) or other scatterers.
Key Features
------------
- **Particle Event Simulation**: Create detailed FSC/SSC signals with customizable particle size and refractive index distributions.
- **Noise and Signal Modeling**: Incorporate realistic noise sources (thermal, shot, dark current) and baseline shifts.
- **Detector Configurations**: Simulate real-world detector behaviors, including saturation and responsivity.
- **Fluorescence Modeling**: Simulate fluorescence signals for labeled particles (e.g., EV surface markers).
- **Visualization Tools**: Generate advanced plots, including density maps and signal traces.
For full documentation and examples, visit the `FlowCyPy Documentation <https://martinpdes.github.io/FlowCyPy/docs/latest/index.html>`_.
Installation
------------
Install FlowCyPy via `pip` or `conda``:
.. code-block:: bash
pip install FlowCyPy
conda install FlowCyPy --channels MartinPdeS
**Requirements**: Python 3.10 or higher with dependencies:
`numpy`, `scipy`, `pint`, `tabulate`, `seaborn`, `MPSPlots`, `PyMieSim`, `pydantic>=2.6.3`
Quick Start
-----------
Simulate a simple flow cytometer experiment:
.. code-block:: python
import numpy as np
from FlowCyPy import Detector, FlowCytometer, ScattererCollection, FlowCell, units, NoiseSetting, GaussianBeam, SignalDigitizer
from FlowCyPy.population import Exosome, Population, distribution
NoiseSetting.include_noises = True
source = GaussianBeam(
numerical_aperture=0.3 * units.AU,
wavelength=200 * units.nanometer,
optical_power=20 * units.milliwatt
)
flow_cell = FlowCell(
source=source,
volume_flow=0.3 * units.microliter / units.second,
flow_area=(10 * units.micrometer) ** 2,
)
scatterer_collection = ScattererCollection(medium_refractive_index=1.33 * units.RIU)
exosome = Exosome(particle_count=5e9 * units.particle / units.milliliter)
custom_population = Population(
name='Pop 0',
particle_count=5e9 * units.particle / units.milliliter,
size=distribution.RosinRammler(characteristic_size=150 * units.nanometer, spread=30),
refractive_index=distribution.Normal(mean=1.44 * units.RIU, std_dev=0.002 * units.RIU)
)
scatterer_collection.add_population(exosome, custom_population)
scatterer_collection.dilute(factor=4)
scatterer_collection.plot()
signal_digitizer = SignalDigitizer(
bit_depth='14bit',
saturation_levels='auto',
sampling_freq=60 * units.megahertz,
)
detector_0 = Detector(
name='forward',
phi_angle=0 * units.degree,
numerical_aperture=1.2 * units.AU,
responsitivity=1 * units.ampere / units.watt,
resistance=50 * units.ohm,
temperature=300 * units.kelvin
)
detector_1 = Detector(
name='side',
phi_angle=90 * units.degree,
numerical_aperture=1.2 * units.AU,
responsitivity=1 * units.ampere / units.watt,
resistance=50 * units.ohm,
temperature=300 * units.kelvin,
)
cytometer = FlowCytometer(
scatterer_collection=scatterer_collection,
signal_digitizer=signal_digitizer,
detectors=[detector_0, detector_1],
flow_cell=flow_cell,
background_power=0.001 * units.milliwatt
)
acquisition = cytometer.get_acquisition(run_time=0.2 * units.millisecond)
acquisition.scatterer.plot(
x='side',
y='forward'
)
acquisition.analog.plot()
triggered_acquisition = acquisition.run_triggering(
threshold=0.2 * units.millivolt,
trigger_detector_name='forward',
max_triggers=35,
pre_buffer=64,
post_buffer=64
)
triggered_acquisition.analog.plot()
Explore more examples in the `FlowCyPy Examples <https://martinpdes.github.io/FlowCyPy/gallery/index.html>`_.
Code structure
--------------
Here is the architecture for a standard workflow using FlowCyPy:
|arch|
Development and Contribution
-----------------------------
Clone the Repository
~~~~~~~~~~~~~~~~~~~~
.. code-block:: bash
git clone https://github.com/MartinPdeS/FlowCyPy.git
cd FlowCyPy
Install Locally
~~~~~~~~~~~~~~~
Install in editable mode with testing and documentation dependencies:
.. code-block:: bash
pip install -e .[testing,documentation] (on linux system)
pip install -e ".[testing,documentation]" (on macOS system)
Run Tests
~~~~~~~~~
Use `pytest` to validate functionality:
.. code-block:: bash
pytest
Build Documentation
~~~~~~~~~~~~~~~~~~~
Build the documentation locally:
.. code-block:: bash
cd docs
make html
Find the documentation in `docs/_build/html`.
Additional Resources
--------------------
- **Documentation**: Full guide and API reference at `FlowCyPy Documentation <https://martinpdes.github.io/FlowCyPy/docs/latest/index.html>`_
- **Examples**: Explore use cases in the `Examples Section <https://martinpdes.github.io/FlowCyPy/gallery/index.html>`_
- **GitHub Repository**: Access the source code and contribute at `FlowCyPy GitHub <https://martinpdes.github.io/FlowCyPy/>`_
Contributions
-------------
Contributions are welcome! If you have suggestions, issues, or would like to collaborate, visit the `GitHub repository <https://github.com/MartinPdeS/FlowCyPy>`_.
Contact
-------
For inquiries or collaboration, contact `Martin Poinsinet de Sivry-Houle <mailto:martin.poinsinet.de.sivry@gmail.com>`_.
.. |logo| image:: https://github.com/MartinPdeS/FlowCyPy/raw/master/docs/images/logo.png
:align: middle
:alt: FlowCyPy Logo
.. |arch| image:: https://github.com/MartinPdeS/FlowCyPy/raw/master/docs/images/architecture.png
:align: middle
:alt: FlowCyPy Logo
.. |python| image:: https://img.shields.io/pypi/pyversions/flowcypy.svg
:alt: Python
:target: https://www.python.org/
.. |docs| image:: https://github.com/martinpdes/flowcypy/actions/workflows/deploy_documentation.yml/badge.svg
:target: https://martinpdes.github.io/FlowCyPy/
:alt: Documentation Status
.. |PyPi| image:: https://badge.fury.io/py/FlowCyPy.svg
:alt: PyPi version
:target: https://badge.fury.io/py/FlowCyPy
.. |PyPi_download| image:: https://img.shields.io/pypi/dm/FlowCyPy?style=plastic&label=PyPi%20downloads&labelColor=hex&color=hex
:alt: PyPI - Downloads
:target: https://pypistats.org/packages/flowcypy
.. |coverage| image:: https://raw.githubusercontent.com/MartinPdeS/FlowCyPy/python-coverage-comment-action-data/badge.svg
:alt: Unittest coverage
:target: https://htmlpreview.github.io/?https://github.com/MartinPdeS/FlowCyPy/blob/python-coverage-comment-action-data/htmlcov/index.html
.. |ci/cd| image:: https://github.com/martinpdes/flowcypy/actions/workflows/deploy_coverage.yml/badge.svg
:alt: Unittest Status
.. |anaconda| image:: https://anaconda.org/martinpdes/flowcypy/badges/version.svg
:alt: Anaconda version
:target: https://anaconda.org/martinpdes/flowcypy
.. |anaconda_download| image:: https://anaconda.org/martinpdes/flowcypy/badges/downloads.svg
:alt: Anaconda downloads
:target: https://anaconda.org/martinpdes/flowcypy
.. |anaconda_date| image:: https://anaconda.org/martinpdes/flowcypy/badges/latest_release_relative_date.svg
:alt: Latest release date
:target: https://anaconda.org/martinpdes/flowcypy
.. |colab| image:: https://colab.research.google.com/assets/colab-badge.svg
:alt: Google Colab
:target: https://colab.research.google.com/github/MartinPdeS/FlowCyPy/blob/master/notebook.ipynb
Raw data
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"keywords": "flow cytometry, light scattering, mie theory, backscatter, rayleigh scattering, biophotonics, nanoparticle, microfluidics, fluorescence, biomedical imaging, spectroscopy, optical simulation, machine learning, signal processing, photonics, cytometry, optical sensors, laser scattering, particle detection, flow analysis",
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"description": "|logo|\n\nFlowCyPy: Flow Cytometer Simulation Tool\n========================================\n\n.. list-table::\n :widths: 10 25 25 25\n :header-rows: 0\n\n * - Meta\n - |python|\n - |docs|\n -\n * - Testing\n - |ci/cd|\n - |coverage|\n - |colab|\n * - PyPi\n - |PyPi|\n - |PyPi_download|\n -\n * - Anaconda\n - |anaconda|\n - |anaconda_download|\n - |anaconda_date|\n\nOverview\n--------\n\n**FlowCyPy** is a cutting-edge Python library designed to simulate flow cytometer experiments. By generating realistic Forward Scatter (FSC) and Side Scatter (SSC) signals, FlowCyPy enables detailed modeling of flow cytometry setups, making it ideal for researchers and engineers working with extracellular vesicles (EVs) or other scatterers.\n\nKey Features\n------------\n\n- **Particle Event Simulation**: Create detailed FSC/SSC signals with customizable particle size and refractive index distributions.\n- **Noise and Signal Modeling**: Incorporate realistic noise sources (thermal, shot, dark current) and baseline shifts.\n- **Detector Configurations**: Simulate real-world detector behaviors, including saturation and responsivity.\n- **Fluorescence Modeling**: Simulate fluorescence signals for labeled particles (e.g., EV surface markers).\n- **Visualization Tools**: Generate advanced plots, including density maps and signal traces.\n\nFor full documentation and examples, visit the `FlowCyPy Documentation <https://martinpdes.github.io/FlowCyPy/docs/latest/index.html>`_.\n\nInstallation\n------------\n\nInstall FlowCyPy via `pip` or `conda``:\n\n.. code-block:: bash\n\n pip install FlowCyPy\n conda install FlowCyPy --channels MartinPdeS\n\n**Requirements**: Python 3.10 or higher with dependencies:\n`numpy`, `scipy`, `pint`, `tabulate`, `seaborn`, `MPSPlots`, `PyMieSim`, `pydantic>=2.6.3`\n\nQuick Start\n-----------\n\nSimulate a simple flow cytometer experiment:\n\n.. code-block:: python\n\n import numpy as np\n from FlowCyPy import Detector, FlowCytometer, ScattererCollection, FlowCell, units, NoiseSetting, GaussianBeam, SignalDigitizer\n from FlowCyPy.population import Exosome, Population, distribution\n\n NoiseSetting.include_noises = True\n\n source = GaussianBeam(\n numerical_aperture=0.3 * units.AU,\n wavelength=200 * units.nanometer,\n optical_power=20 * units.milliwatt\n )\n\n flow_cell = FlowCell(\n source=source,\n volume_flow=0.3 * units.microliter / units.second,\n flow_area=(10 * units.micrometer) ** 2,\n )\n\n scatterer_collection = ScattererCollection(medium_refractive_index=1.33 * units.RIU)\n\n exosome = Exosome(particle_count=5e9 * units.particle / units.milliliter)\n\n custom_population = Population(\n name='Pop 0',\n particle_count=5e9 * units.particle / units.milliliter,\n size=distribution.RosinRammler(characteristic_size=150 * units.nanometer, spread=30),\n refractive_index=distribution.Normal(mean=1.44 * units.RIU, std_dev=0.002 * units.RIU)\n )\n\n scatterer_collection.add_population(exosome, custom_population)\n\n scatterer_collection.dilute(factor=4)\n\n scatterer_collection.plot()\n\n signal_digitizer = SignalDigitizer(\n bit_depth='14bit',\n saturation_levels='auto',\n sampling_freq=60 * units.megahertz,\n )\n\n detector_0 = Detector(\n name='forward',\n phi_angle=0 * units.degree,\n numerical_aperture=1.2 * units.AU,\n responsitivity=1 * units.ampere / units.watt,\n resistance=50 * units.ohm,\n temperature=300 * units.kelvin\n )\n\n detector_1 = Detector(\n name='side',\n phi_angle=90 * units.degree,\n numerical_aperture=1.2 * units.AU,\n responsitivity=1 * units.ampere / units.watt,\n resistance=50 * units.ohm,\n temperature=300 * units.kelvin,\n )\n\n cytometer = FlowCytometer(\n scatterer_collection=scatterer_collection,\n signal_digitizer=signal_digitizer,\n detectors=[detector_0, detector_1],\n flow_cell=flow_cell,\n background_power=0.001 * units.milliwatt\n )\n\n acquisition = cytometer.get_acquisition(run_time=0.2 * units.millisecond)\n\n acquisition.scatterer.plot(\n x='side',\n y='forward'\n )\n\n acquisition.analog.plot()\n\n triggered_acquisition = acquisition.run_triggering(\n threshold=0.2 * units.millivolt,\n trigger_detector_name='forward',\n max_triggers=35,\n pre_buffer=64,\n post_buffer=64\n )\n\n triggered_acquisition.analog.plot()\n\nExplore more examples in the `FlowCyPy Examples <https://martinpdes.github.io/FlowCyPy/gallery/index.html>`_.\n\n\n\nCode structure\n--------------\n\nHere is the architecture for a standard workflow using FlowCyPy:\n\n\n|arch|\n\n\n\nDevelopment and Contribution\n-----------------------------\n\nClone the Repository\n~~~~~~~~~~~~~~~~~~~~\n\n.. code-block:: bash\n\n git clone https://github.com/MartinPdeS/FlowCyPy.git\n cd FlowCyPy\n\nInstall Locally\n~~~~~~~~~~~~~~~\n\nInstall in editable mode with testing and documentation dependencies:\n\n.. code-block:: bash\n\n pip install -e .[testing,documentation] (on linux system)\n pip install -e \".[testing,documentation]\" (on macOS system)\n\nRun Tests\n~~~~~~~~~\n\nUse `pytest` to validate functionality:\n\n.. code-block:: bash\n\n pytest\n\nBuild Documentation\n~~~~~~~~~~~~~~~~~~~\n\nBuild the documentation locally:\n\n.. code-block:: bash\n\n cd docs\n make html\n\nFind the documentation in `docs/_build/html`.\n\nAdditional Resources\n--------------------\n\n - **Documentation**: Full guide and API reference at `FlowCyPy Documentation <https://martinpdes.github.io/FlowCyPy/docs/latest/index.html>`_\n - **Examples**: Explore use cases in the `Examples Section <https://martinpdes.github.io/FlowCyPy/gallery/index.html>`_\n - **GitHub Repository**: Access the source code and contribute at `FlowCyPy GitHub <https://martinpdes.github.io/FlowCyPy/>`_\n\nContributions\n-------------\n\nContributions are welcome! If you have suggestions, issues, or would like to collaborate, visit the `GitHub repository <https://github.com/MartinPdeS/FlowCyPy>`_.\n\nContact\n-------\n\nFor inquiries or collaboration, contact `Martin Poinsinet de Sivry-Houle <mailto:martin.poinsinet.de.sivry@gmail.com>`_.\n\n.. |logo| image:: https://github.com/MartinPdeS/FlowCyPy/raw/master/docs/images/logo.png\n :align: middle\n :alt: FlowCyPy Logo\n\n.. |arch| image:: https://github.com/MartinPdeS/FlowCyPy/raw/master/docs/images/architecture.png\n :align: middle\n :alt: FlowCyPy Logo\n\n.. |python| image:: https://img.shields.io/pypi/pyversions/flowcypy.svg\n :alt: Python\n :target: https://www.python.org/\n\n.. |docs| image:: https://github.com/martinpdes/flowcypy/actions/workflows/deploy_documentation.yml/badge.svg\n :target: https://martinpdes.github.io/FlowCyPy/\n :alt: Documentation Status\n\n.. |PyPi| image:: https://badge.fury.io/py/FlowCyPy.svg\n :alt: PyPi version\n :target: https://badge.fury.io/py/FlowCyPy\n\n.. |PyPi_download| image:: https://img.shields.io/pypi/dm/FlowCyPy?style=plastic&label=PyPi%20downloads&labelColor=hex&color=hex\n :alt: PyPI - Downloads\n :target: https://pypistats.org/packages/flowcypy\n\n.. |coverage| image:: https://raw.githubusercontent.com/MartinPdeS/FlowCyPy/python-coverage-comment-action-data/badge.svg\n :alt: Unittest coverage\n :target: https://htmlpreview.github.io/?https://github.com/MartinPdeS/FlowCyPy/blob/python-coverage-comment-action-data/htmlcov/index.html\n\n.. |ci/cd| image:: https://github.com/martinpdes/flowcypy/actions/workflows/deploy_coverage.yml/badge.svg\n :alt: Unittest Status\n\n.. |anaconda| image:: https://anaconda.org/martinpdes/flowcypy/badges/version.svg\n :alt: Anaconda version\n :target: https://anaconda.org/martinpdes/flowcypy\n\n.. |anaconda_download| image:: https://anaconda.org/martinpdes/flowcypy/badges/downloads.svg\n :alt: Anaconda downloads\n :target: https://anaconda.org/martinpdes/flowcypy\n\n.. |anaconda_date| image:: https://anaconda.org/martinpdes/flowcypy/badges/latest_release_relative_date.svg\n :alt: Latest release date\n :target: https://anaconda.org/martinpdes/flowcypy\n\n.. |colab| image:: https://colab.research.google.com/assets/colab-badge.svg\n :alt: Google Colab\n :target: https://colab.research.google.com/github/MartinPdeS/FlowCyPy/blob/master/notebook.ipynb",
"bugtrack_url": null,
"license": "MIT License\n \n Copyright (c) 2020 Martin de Sivry\n \n Permission is hereby granted, free of charge, to any person obtaining a copy\n of this software and associated documentation files (the \"Software\"), to deal\n in the Software without restriction, including without limitation the rights\n to use, copy, modify, merge, publish, distribute, sublicense, and/or sell\n copies of the Software, and to permit persons to whom the Software is\n furnished to do so, subject to the following conditions:\n \n The above copyright notice and this permission notice shall be included in all\n copies or substantial portions of the Software.\n \n THE SOFTWARE IS PROVIDED \"AS IS\", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR\n IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,\n FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE\n AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER\n LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,\n OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE\n SOFTWARE.\n ",
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