# Cyclic ImmunoFluorescence Automatic Analysis Pipeline
This pipeline takes as input a set of cyclic immunofluorescence (cycIF) images and performs the following operations:
- Registration
- Nuclei segmentation (using CellPose or own trained Mask R-CNN model)
- Background subtraction
- Compute each marker exclusiveness to be used with Restore
- Use Restore when possible for automatic gating (optionnal)
- Features extraction
- Cell type computation
- Automatic visualization using Napari
- Quality control (tissue loss and Restore based)
For installation, more information/details and full examples with code and data, visit:
https://www.thibault.biz/Research/cycIFAAP/cycIFAAP.html
Raw data
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