<p align="center">
<a href="https://github.com/jlsteenwyk/orthofisher">
<img src="https://raw.githubusercontent.com/JLSteenwyk/orthofisher/master/docs/_static/img/orthofisher_full_logo.png" alt="Logo" width="400">
</a>
<p align="center">
<a href="https://jlsteenwyk.com/orthofisher/">Docs</a>
·
<a href="https://github.com/jlsteenwyk/orthofisher/issues">Report Bug</a>
·
<a href="https://github.com/jlsteenwyk/orthofisher/issues">Request Feature</a>
</p>
<p align="center">
<a href="https://github.com/JLSteenwyk/orthofisher/actions" alt="Build">
<img src="https://img.shields.io/github/actions/workflow/status/JLSteenwyk/orthofisher/ci.yml?branch=main">
</a>
<a href="https://codecov.io/gh/jlsteenwyk/orthofisher" alt="Coverage">
<img src="https://codecov.io/gh/JLSteenwyk/orthofisher/branch/main/graph/badge.svg?token=7LYMLHDL9D"/>
</a>
<a href="https://github.com/jlsteenwyk/orthofisher/graphs/contributors" alt="Contributors">
<img src="https://img.shields.io/github/contributors/jlsteenwyk/orthofisher">
</a>
<a href="https://twitter.com/intent/follow?screen_name=jlsteenwyk" alt="Author Twitter">
<img src="https://img.shields.io/twitter/follow/jlsteenwyk?style=social&logo=twitter"
alt="follow on Twitter">
</a>
<br />
<a href="https://pepy.tech/badge/orthofisher">
<img src="https://static.pepy.tech/personalized-badge/orthofisher?period=total&units=international_system&left_color=grey&right_color=blue&left_text=PyPi%20Downloads">
</a>
<a href="https://lbesson.mit-license.org/" alt="License">
<img src="https://img.shields.io/badge/License-MIT-blue.svg">
</a>
<a href="https://pypi.org/project/orthofisher/" alt="PyPI - Python Version">
<img src="https://img.shields.io/pypi/pyversions/orthofisher">
</a>
<a href="https://academic.oup.com/g3journal/article/11/9/jkab250/6321954">
<img src="https://zenodo.org/badge/DOI/10.1093/g3journal/jkab250.svg">
</a>
</p>
</p>
<br />
Orthofisher conducts automated and high-throughout identification of a predetermined set of orthologs, which can be used for phylgenomics, gene family copy number determination and more!<br /><br />
If you found orthofisher useful, please cite *orthofisher: a broadly applicable tool for automated gene identification and retrieval*. Steenwyk & Rokas 2021, G3 Genes|Genomes|Genetics. doi: [10.1093/g3journal/jkab250](https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkab250/6321954?login=true).
<br /><br />
---
<br />
## Guide
[Quick Start](#quick-start)<br />
[Performance Assessment](#performance-assessment)<br />
[FAQ](#faq)
<br />
---
<br/>
## Quick Start
For detailed instructions on usage and a tutorial, please see the [online documentation](https://jlsteenwyk.com/orthofisher/).
<br/>
### 1) Prerequisite
Before installing orthofisher, please first install [HMMER3](http://hmmer.org/documentation.html) and add the HMMER to your .bashrc path. For example, my .bashrc has the following:
```shell
export PATH=$PATH:/home/steenwj/SOFTWARE/hmmer-3.1b2-linux-intel-x86_64/binaries
```
<br/>
### 2) Install orthofisher
**If you are having trouble installing orthofisher, please contact the lead developer, Jacob L. Steenwyk, via [email](https://jlsteenwyk.com/contact.html) or [twitter](https://twitter.com/jlsteenwyk) to get help.**
<br/>
To install via *anaconda*, execute the follwoing command:
``` shell
conda install -c jlsteenwyk orthofisher
```
Visit here for more information: https://anaconda.org/jlsteenwyk/orthofisher
<br/>
To install via *pip*, execute the follwoing command:
```shell
pip install orthofisher
```
<br />
To install from source, execute the follwoing command:
```shell
# download
git clone https://github.com/JLSteenwyk/orthofisher.git
# change dir
cd orthofisher/
# install
make install
```
<br/>
If you run into software dependency issues, install orthofisher in a virtual environment. To do so, create your virtual environment with the following command:
```shell
# create virtual environment
python -m venv .venv
# activate virtual environment
source .venv/bin/activate
```
Next, install the software using your preferred method above. Thereafter, you will be able to use orthofisher.
To deactivate your virtual environment, use the following command:
```shell
# deactivate virtual environment
deactivate
```
**Note, the virtual environment must be activated to use *orthofisher*.**
<br />
---
<br />
## Performance Assessment
Using 1,530 sequence similarity searches across six model eukaryotic proteomes, the performance of orthofisher was compared to results obtained from BUSCO. Examination of precision and recall revealed near perfect performance. More specifically, orthofisher had a <strong>recall of 1.0 and precision of 0.99</strong>. Precision is less than 1.0 because priors of expected sequence length and sequence similarity scores--which are not implemented in orthofisher--resulted in more missing genes in the BUSCO pipeline than the orthofisher pipeline.
<br />
---
<br />
## FAQ
<br />
<strong>I am having trouble installing orthofisher, what should I do?</strong>
Please install orthofisher using a virtual environment as described in the installation instructions. If you are still running into issues after installing in a virtual environment, please contact Jacob L. Steenwyk via [email](https://jlsteenwyk.com/contact.html) or [twitter](https://twitter.com/jlsteenwyk).
<br />
---
<br />
### orthofisher is developed and maintained by [Jacob Steenwyk](https://jlsteenwyk.github.io/)
Raw data
{
"_id": null,
"home_page": "https://github.com/JLSteenwyk/orthofisher.git",
"name": "orthofisher",
"maintainer": null,
"docs_url": null,
"requires_python": null,
"maintainer_email": null,
"keywords": null,
"author": "Jacob L. Steenwyk",
"author_email": "jlsteenwyk@gmail.com",
"download_url": "https://files.pythonhosted.org/packages/b7/6c/471aa1953a553047df38a8186b7e260c4d7949a9bb6feef52d9709d34558/orthofisher-1.1.1.tar.gz",
"platform": null,
"description": "<p align=\"center\">\n <a href=\"https://github.com/jlsteenwyk/orthofisher\">\n <img src=\"https://raw.githubusercontent.com/JLSteenwyk/orthofisher/master/docs/_static/img/orthofisher_full_logo.png\" alt=\"Logo\" width=\"400\">\n </a>\n <p align=\"center\">\n <a href=\"https://jlsteenwyk.com/orthofisher/\">Docs</a>\n \u00b7\n <a href=\"https://github.com/jlsteenwyk/orthofisher/issues\">Report Bug</a>\n \u00b7\n <a href=\"https://github.com/jlsteenwyk/orthofisher/issues\">Request Feature</a>\n </p>\n <p align=\"center\">\n <a href=\"https://github.com/JLSteenwyk/orthofisher/actions\" alt=\"Build\">\n <img src=\"https://img.shields.io/github/actions/workflow/status/JLSteenwyk/orthofisher/ci.yml?branch=main\">\n </a>\n <a href=\"https://codecov.io/gh/jlsteenwyk/orthofisher\" alt=\"Coverage\">\n <img src=\"https://codecov.io/gh/JLSteenwyk/orthofisher/branch/main/graph/badge.svg?token=7LYMLHDL9D\"/>\n </a>\n <a href=\"https://github.com/jlsteenwyk/orthofisher/graphs/contributors\" alt=\"Contributors\">\n <img src=\"https://img.shields.io/github/contributors/jlsteenwyk/orthofisher\">\n </a>\n <a href=\"https://twitter.com/intent/follow?screen_name=jlsteenwyk\" alt=\"Author Twitter\">\n <img src=\"https://img.shields.io/twitter/follow/jlsteenwyk?style=social&logo=twitter\"\n alt=\"follow on Twitter\">\n </a>\n <br />\n <a href=\"https://pepy.tech/badge/orthofisher\">\n <img src=\"https://static.pepy.tech/personalized-badge/orthofisher?period=total&units=international_system&left_color=grey&right_color=blue&left_text=PyPi%20Downloads\">\n </a>\n <a href=\"https://lbesson.mit-license.org/\" alt=\"License\">\n <img src=\"https://img.shields.io/badge/License-MIT-blue.svg\">\n </a>\n <a href=\"https://pypi.org/project/orthofisher/\" alt=\"PyPI - Python Version\">\n <img src=\"https://img.shields.io/pypi/pyversions/orthofisher\">\n </a>\n <a href=\"https://academic.oup.com/g3journal/article/11/9/jkab250/6321954\">\n <img src=\"https://zenodo.org/badge/DOI/10.1093/g3journal/jkab250.svg\">\n </a>\n </p>\n</p>\n\n<br />\n\nOrthofisher conducts automated and high-throughout identification of a predetermined set of orthologs, which can be used for phylgenomics, gene family copy number determination and more!<br /><br />\n\nIf you found orthofisher useful, please cite *orthofisher: a broadly applicable tool for automated gene identification and retrieval*. Steenwyk & Rokas 2021, G3 Genes|Genomes|Genetics. doi: [10.1093/g3journal/jkab250](https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkab250/6321954?login=true).\n<br /><br />\n\n---\n\n<br />\n\n## Guide\n[Quick Start](#quick-start)<br />\n[Performance Assessment](#performance-assessment)<br />\n[FAQ](#faq)\n\n<br />\n\n---\n\n<br/>\n\n## Quick Start\n\nFor detailed instructions on usage and a tutorial, please see the [online documentation](https://jlsteenwyk.com/orthofisher/).\n\n<br/>\n\n### 1) Prerequisite\nBefore installing orthofisher, please first install [HMMER3](http://hmmer.org/documentation.html) and add the HMMER to your .bashrc path. For example, my .bashrc has the following:\n\n```shell\nexport PATH=$PATH:/home/steenwj/SOFTWARE/hmmer-3.1b2-linux-intel-x86_64/binaries\n```\n\n<br/>\n\n### 2) Install orthofisher\n\n**If you are having trouble installing orthofisher, please contact the lead developer, Jacob L. Steenwyk, via [email](https://jlsteenwyk.com/contact.html) or [twitter](https://twitter.com/jlsteenwyk) to get help.**\n\n<br/>\n\nTo install via *anaconda*, execute the follwoing command:\n``` shell\nconda install -c jlsteenwyk orthofisher\n```\nVisit here for more information: https://anaconda.org/jlsteenwyk/orthofisher\n\n<br/>\n\nTo install via *pip*, execute the follwoing command:\n```shell\npip install orthofisher\n```\n\n<br />\n\nTo install from source, execute the follwoing command:\n```shell\n# download\ngit clone https://github.com/JLSteenwyk/orthofisher.git\n# change dir\ncd orthofisher/\n# install\nmake install\n```\n\n<br/>\n\nIf you run into software dependency issues, install orthofisher in a virtual environment. To do so, create your virtual environment with the following command: \n```shell\n# create virtual environment\npython -m venv .venv\n# activate virtual environment\nsource .venv/bin/activate\n```\n\nNext, install the software using your preferred method above. Thereafter, you will be able to use orthofisher.\n\nTo deactivate your virtual environment, use the following command:\n```shell\n# deactivate virtual environment\ndeactivate\n```\n**Note, the virtual environment must be activated to use *orthofisher*.**\n\n<br />\n\n---\n\n<br />\n\n## Performance Assessment\nUsing 1,530 sequence similarity searches across six model eukaryotic proteomes, the performance of orthofisher was compared to results obtained from BUSCO. Examination of precision and recall revealed near perfect performance. More specifically, orthofisher had a <strong>recall of 1.0 and precision of 0.99</strong>. Precision is less than 1.0 because priors of expected sequence length and sequence similarity scores--which are not implemented in orthofisher--resulted in more missing genes in the BUSCO pipeline than the orthofisher pipeline.\n\n<br />\n\n---\n\n<br />\n\n## FAQ\n\n<br />\n\n<strong>I am having trouble installing orthofisher, what should I do?</strong>\n\nPlease install orthofisher using a virtual environment as described in the installation instructions. If you are still running into issues after installing in a virtual environment, please contact Jacob L. Steenwyk via [email](https://jlsteenwyk.com/contact.html) or [twitter](https://twitter.com/jlsteenwyk).\n\n<br />\n\n---\n\n<br />\n\n### orthofisher is developed and maintained by [Jacob Steenwyk](https://jlsteenwyk.github.io/)\n\n",
"bugtrack_url": null,
"license": null,
"summary": "orthofisher",
"version": "1.1.1",
"project_urls": {
"Homepage": "https://github.com/JLSteenwyk/orthofisher.git"
},
"split_keywords": [],
"urls": [
{
"comment_text": "",
"digests": {
"blake2b_256": "f6c58b2c6ac112fe842df5776a63ba58dbde2791994acb842c7b4c693e8829e4",
"md5": "12ce1fccabe32b82547546b2f7774d32",
"sha256": "c206ee7daa2afae18a207ddf4c41466d3b212a0dafa09dded4ba62afdfcb3cac"
},
"downloads": -1,
"filename": "orthofisher-1.1.1-py2.py3-none-any.whl",
"has_sig": false,
"md5_digest": "12ce1fccabe32b82547546b2f7774d32",
"packagetype": "bdist_wheel",
"python_version": "py2.py3",
"requires_python": null,
"size": 12754,
"upload_time": "2024-06-03T22:42:36",
"upload_time_iso_8601": "2024-06-03T22:42:36.248694Z",
"url": "https://files.pythonhosted.org/packages/f6/c5/8b2c6ac112fe842df5776a63ba58dbde2791994acb842c7b4c693e8829e4/orthofisher-1.1.1-py2.py3-none-any.whl",
"yanked": false,
"yanked_reason": null
},
{
"comment_text": "",
"digests": {
"blake2b_256": "b76c471aa1953a553047df38a8186b7e260c4d7949a9bb6feef52d9709d34558",
"md5": "45c323cec5426392f2535036403d0c5b",
"sha256": "0a71d21864db9e104eefd636e0185969fb5da25a01770b578966aca75517c046"
},
"downloads": -1,
"filename": "orthofisher-1.1.1.tar.gz",
"has_sig": false,
"md5_digest": "45c323cec5426392f2535036403d0c5b",
"packagetype": "sdist",
"python_version": "source",
"requires_python": null,
"size": 11312,
"upload_time": "2024-06-03T22:42:37",
"upload_time_iso_8601": "2024-06-03T22:42:37.427043Z",
"url": "https://files.pythonhosted.org/packages/b7/6c/471aa1953a553047df38a8186b7e260c4d7949a9bb6feef52d9709d34558/orthofisher-1.1.1.tar.gz",
"yanked": false,
"yanked_reason": null
}
],
"upload_time": "2024-06-03 22:42:37",
"github": true,
"gitlab": false,
"bitbucket": false,
"codeberg": false,
"github_user": "JLSteenwyk",
"github_project": "orthofisher",
"travis_ci": false,
"coveralls": false,
"github_actions": true,
"requirements": [],
"lcname": "orthofisher"
}
JSON
