scipion-em-gctf


Namescipion-em-gctf JSON
Version 3.1.3 PyPI version JSON
download
home_pagehttps://github.com/scipion-em/scipion-em-gctf
SummaryPlugin to use Gctf program within the Scipion framework
upload_time2023-11-19 13:40:29
maintainer
docs_urlNone
authorGrigory Sharov, J.M. De la Rosa Trevin
requires_python
license
keywords scipion electron-microscopy cryo-em structural-biology image-processing scipion-3.0
VCS
bugtrack_url
requirements No requirements were recorded.
Travis-CI No Travis.
coveralls test coverage No coveralls.
            ===========
Gctf plugin
===========

This plugin provides a wrapper for `Gctf <https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/>`_ program.

.. image:: https://img.shields.io/pypi/v/scipion-em-gctf.svg
        :target: https://pypi.python.org/pypi/scipion-em-gctf
        :alt: PyPI release

.. image:: https://img.shields.io/pypi/l/scipion-em-gctf.svg
        :target: https://pypi.python.org/pypi/scipion-em-gctf
        :alt: License

.. image:: https://img.shields.io/pypi/pyversions/scipion-em-gctf.svg
        :target: https://pypi.python.org/pypi/scipion-em-gctf
        :alt: Supported Python versions

.. image:: https://img.shields.io/sonar/quality_gate/scipion-em_scipion-em-gctf?server=https%3A%2F%2Fsonarcloud.io
        :target: https://sonarcloud.io/dashboard?id=scipion-em_scipion-em-gctf
        :alt: SonarCloud quality gate

.. image:: https://img.shields.io/pypi/dm/scipion-em-gctf
        :target: https://pypi.python.org/pypi/scipion-em-gctf
        :alt: Downloads

Installation
------------

You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:

a) Stable version

   .. code-block::

      scipion installp -p scipion-em-gctf

b) Developer's version

   * download repository

   .. code-block::

      git clone -b devel https://github.com/scipion-em/scipion-em-gctf.git

   * install

   .. code-block::

      scipion installp -p /path/to/scipion-em-gctf --devel

- Gctf binaries will be installed automatically with the plugin, but you can also link an existing installation. Default installation path assumed is ``software/em/gctf-1.18``, if you want to change it, set *GCTF_HOME* in ``scipion.conf`` file to the folder where the Gctf is installed. Depending on your CUDA version and GPU card compute capability you might want to change the default binary from ``Gctf_v1.18_sm30-75_cu10.1`` to a different one by explicitly setting *GCTF* variable.
- If you need to use CUDA different from the one used during Scipion installation (defined by *CUDA_LIB*), you can add *GCTF_CUDA_LIB* variable to the config file. Various binaries can be downloaded from the official Gctf website.

To check the installation, simply run one of the following Scipion tests: 

.. code-block::

   scipion test gctf.tests.test_protocols_gctf.TestGctfRefine
   scipion test gctf.tests.test_protocols_gctf.TestGctf
   scipion tests gctf.tests.test_protocols_gctf_ts.TestGctfTs

Supported versions
------------------

1.06 and 1.18.

1.18 is a special version designed for VPP data, it does not support local/movie CTF refinement or validation options. 

Protocols
---------

* ctf estimation
* ctf refinement
* tilt-series gctf

References
----------

1. Zhang K. (2016). Gctf: Real-time CTF determination and correction. JSB, 193: 1-12.



            

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